学术论文
-
Download Resource
发布日期:2016年03月14日Examples of the datasets of our research are provided. This file contains two datasets.
The first one consists of two groups of data, the self-skin transplantation group (the first group) and the allogenic skin transplantation group (the second group).The second one includes four groups of data, which are manually labeled. The deformation intensity of the four groups increases along with Normal, Slight, Moderate and Drastic.
Experiment process:For Dataset I, healthy Balb/C male mice were used as both hosts and donors in the first group, and in the other group, healthy Balb/C male mice and healthy C57BL/6 male mice were used as hosts and donors respectively. While there is only one group, in which healthy Balb/C male mice and healthy C57BL/6 male mice were used as hosts and donors, in Dataset II. Both kinds of mice were 6 to 8 weeks old with weight of 20 to 22 grams, and were obtained from Graduate School of the Academy of Military Medical Sciences (Beijing).The skin transplantation was conducted as following steps. Firstly, the donors were killed by breaking their necks, and then their torso hair was shaved. The graft skin was from their back and cut into pieces with size of 1.5cm * 1.5cm. Then it was stored in the plates with sterile PBS (Phosphate Buffer Saline). Afterward, the hosts were anaesthetized by sodium pentobarbital (70 ug/g of weight), and their back was shaved and cleaned with alcohol. The surgical area was disinfected with iodine and removed. Subsequently, the graft skin was positioned on the hosts, and covered by Vaseline gauze to prevent sticking and dehydration. Finally, the graft skin was fixed with band aid. After the surgery, the hosts are raised individually in sterile cages in ventilated environment.
Lymphocytes were obtained from the peripheral blood samples from the tail vein of both groups 14 days after the skin transplantation for Dataset I, while 7 days after the skin transplantation Dataset II was collected. The lymphocytes were observed with a phase contrast microscopy (Olympus BX51, 0.3um resolution, 1000* magnification) and recorded into 20-30 seconds with 25 frames per second.